目的构建钩藤药材中钩藤碱、异钩藤碱、柯诺辛及柯诺辛b四种生物碱对谷氨酸酯诱导PC12细胞氧化应激抑制作用的谱-效关系模型。方法在获得钩藤药材中钩藤碱、异钩藤碱、柯诺辛及柯诺辛b四种生物碱对谷氨酸酯诱导的PC12氧化应激的抑制作用的谱-效关系基础上,利用SPSS 15.0软件进行分析,建立谱-效关系模型。结果钩藤碱和柯诺辛对PC12细胞氧化应激有显著的抑制作用,其谱-效方程为:y=44.301+0.255X1+0.356X3,经7批药材验证,谱-效关系式计算值与实际值的偏差率不超过10%。结论该谱-效关系模式可用于预测钩藤对谷氨酸酯诱导的PC12细胞氧化应激的抑制作用。Objective To establish chromatogram-effect model based on inhibitory effect of four alkaloids( rhynchophyline,isorhynchophylline,corynoxine,corynoxine B) on oxidative stress of glutamate-induced PC12 cells.Methods Based on the chromatogram-effect relation of inhibitory effect of four alkaloids on the oxidative stress of glutamate-induced PC12 cells,SPSS 15.0 was used to analyse data.Results Oxidative stress of PC12 cells was significantly inhibited by rhynchophyline and corynoxine.The spectrum efficient equation was,Y = 44.301 + 0.255X 1 + 0.356X 3( P <0.05).The deviation of predictive value and true value was no more than 10% by data validation from seven samples.Conclusions The chromatogram-effect model can be used to predict the effect of uncaria macrophylla wall against the oxidative stress of glutamate-induced PC12 cells
