甘油脱氢酶(gdH)可以催化甘油生成二羟基丙酮.为了进一步探索gdH的活性结构中心,提高gdH的活性,用Mg2+、Mn2+、bA2+、CA2+、zn2+、Cu2+、Sr2+、nI2+、Cd2+和CO2+等10种金属离子对gdH进行活性中心金属离子置换修饰,筛选出具有修饰效果的离子为Mg2+、Mn2+和bA2+,修饰后其最大酶活力分别是初始酶活力的3.4,2.0和4.7倍.通过均匀设计对3种金属离子的修饰条件进行了优化,优化后测定的酶活力分别是未修饰gdH酶活力值3.17 u/Ml的14.9,11.3和12.4倍,极大地提高了gdH的酶活力.Glycerol dehydrogenase(GDH) can catalyze the interconversion of glycerol and dihydroxyacetone.In order to improve the activity and explore the active central site of GDH,10 kinds of metal ions such as Mg2+,Mn2+,Ba2+,Ca2+,Zn2+,Cu2+,Sr2+,Ni2+,Cd2+and Co2+ were used to do chemical modification on GDH.The results approved that the activity of GDH was significantly increased by Mg2+,Mn2+ and Ba2+ while the special activity of GDH was 3.4,2.0 and 4.7 folds respectively of the control′s after modification.Meanwhile,the activity effects of Mg2+,Ba2+ and Mn2+ on GDH showed that the GDH was successfully modificated by Mg2+,Ba2+ and Mn2+.The modificated condition was optimized with the uniform design.Compared to the original activity of GDH 3.17 U/mL,the activity of GDH modified by Mg2+,Ba2+ and Mn2+ was improved separately as 14.9,11.3 and 12.4 folds after optimization.国家高技术研究发展计划(863)项目(2006AA020103);福建省高校产学合作科技重大项目(2010H6023
