将所提出的光谱检测新技术应用于染料标记蛋白质的分析测定， 所得光谱图形与吸收光谱相似， 检测限比相应的分光光度法测定降低1 个数量级， 并利用等摩尔连续变化法和摩尔比法测定了染料与蛋白质的配位比.The novel spectrometric technique proposed applied to study labeling dyes.The spectral profiles gained by this technique were all similar to the corresponding UV-Vis absorption spectra,and the detection limits of dyes by this method were10 times lower than those by spectrophotometry.国家自然科学基金!（29745002
