采用微液滴操作法在厦门海域分离培养了一株海洋鞭毛虫C6,运用显微镜观察和18SrdnA基因技术鉴定其种类,并将其与gEnbAnk中已知的14种海洋鞭毛虫18SrdnA序列比较,用nJ法和uPgMA法构建系统发育树.通过PCr扩增获得1 774bP 18SrdnA序列,与nCbI已登陆的其他同源序列进行系统分析,结果显示此海洋鞭毛虫属于领鞭毛虫(CHOAnOflAgEllATES).与已知种MOnOSIgA brEVICOllIS的相似性为99%,为进一步研究该种鞭毛虫提供了遗传背景,但要明确其种类还需其他研究佐证.Using microscope and 18 SrDNA techniques,a strain of nanoflagellate(C6)separated in Xiamen Bay was identified and analyzed.One 1 774 bp fragment of 18 SrDNA was amplified from the strain C6.Homology analysis between the yielded sequence and the 18 SrDNA sequences accessed in the National Center for Biotechnology Information(NCBI)from other strains showed that C6 belonged to choanoflagellates,which shared 99% homologue with the known species of Monosiga brevicollis.Choanoflagellates play a very important role in biological evolution and marine ecology,however,molecular phylogenetic studies within choanoflagellates are still extremely limited.According to results of morphological and DNA sequence analysis,we consider that C6 should be Monosiga brevicollis,but more collaborations are still required for specific identification.This study provides basic genetic information for further study of physiological characteristics and ecological functions of the strain C6 in Xiamen Bay.国家自然科学基金(41376131); 国家重点基础研究发展计划(973计划)(2011CB409804
