于2008年7月在黄、渤海区域采集了17个漂移浒苔样品,结合3个本地种,对其脂肪酸组成进行了gC-MS分析。结果表明,所有浒苔中优势脂肪酸为十六碳酸,相对含量在27.82%—43.27%之间。藻体中还含有丰富的PufA,主要为9,12,15-十八碳三烯酸、6,9,12-十八碳三烯酸、9,12-十八碳二烯酸、8,11,14-二十碳三烯酸,另外还含有EPA不饱和脂肪酸。以欧式距离平方为距离测量技术,类间距用平均链锁法,对20个不同地理位置的浒苔样品进行聚类分析,结果分为4大类,所有漂浮浒苔聚为第一类,青岛和连云港的本地种分别聚为另三类,说明青岛近海漂浮的浒苔不是青岛本地生浒苔,而是从外海漂移过来的,第一类的聚类结果显示了浒苔的漂移路径。结果表明,藻类脂肪酸的系统聚类分析为海洋绿藻分类与量化评价提供了一种好方法。Fatty acids composition of 20 Enteromorpha prolifera samples from the Yellow Sea and the Bohai Sea were determined with GC-MS.The result indicates that C16 are the predominant fatty acid,taking 27.82% to 43.27%.C18:2(cis,cis-9,12)(n-6),C18:3(gamma)(all cis-6,9,12)(n-6),C18:3(alpha)(all cis-9,12,15)(n-3) and EPA are major polyunsaturated fatty acids(PUFAs) in E.prolifera.The samples were numerically classified in fatty acid composition using Squared Euclidean distance and between-group linkage method as clustering algrithm.The 20 samples were divided into four groups by cluster analysis.All the floating E.prolifera samples are placed in Group I,and other native species in Qingdao and Lianyungang are classified into other three groups.The floating E.prolifera that bloomed in Qingdao coastal area in 2008 were not orininated from local native species but from the open sea.Hierarchical cluster analysis was proved to be a good method of quantitive evaluation and classification of marine green algae.海洋公益性行业科研专项;200805039号;国家海洋局第一海洋研究所基本科研业务费专项资金项目;2008G47号、2008T32号;国家自然科学基金资助项目;20602009号、40776098号;海洋863项目;2007AA09Z435
