本文用￣14C示踪法测定日本对虾糠-3幼体对褶皱臂尾轮虫，卤虫和亚心形扁藻的摄食率、吸收率和利用率；以￣3H-葡萄糖和￣14C-苯丙氨酸作示踪剂研究人工配合饵料中葡萄糖和氨基酸在日本对虾体内转运和分布。实验结果表明褶皱臂尾轮虫是日本对虾糠-3幼体最适的动物性饵料之一。糠-3幼体对卤虫无节幼体摄食与利用率不高，但适量投放23只/Ml密度的卤虫无节幼体作补充饵料以满足幼体对食物日益增长的需要，有利于幼体的生长。虽然糠-3幼体食性以动物性为主，但单独投放适宜密度亚心形扁藻也能摄食。其利用率可达40%左右。日本对虾的营养物质主要通过中肠被吸收，经血液循环被输送到组织器官。氨基酸更多参与对虾组织构成细胞的成分代谢活动，而葡萄糖则是对虾能量代谢活动的主要来源。Feeding rates,absorption rates and utilization eFFiciency of Brachionus plicatilis,Artemiasalina and Platymonas subcordiFormis by Penaeus japonicus Bate larvae(M_3)were deter-mined with￣14C-traeer method.Transportation and distribution of glucose and amino acids of artiFicial diets in Penaeus japonicus were studied with￣3H-glucose and￣14C-phenylalanine as the tracers.Experimental results suggested that Brachionus plicatilis was one of the most suitable diets For the growth of Penaeus japonicus at mysis-3 stage.Artemia salina could be used as a supplemental Food at denisties of 2-3 individuals/ ml to improve the growth of Penaeus larvae。The unicellular alga, Platymonas subcordiFormis,could also be utilized by the mysis-3 stage larvae when provided alone, and the utilization eFFiciency was about40%。Nutrients were mainly absorbed through mid-gut gland and mid-gut,and then trans ported into tissues and organs by means of blood circulation。Amino acids secm to be activ;in cell metabolism of tissues in Penaeus japonicus,whereas glucose is used as a major source For energy metabolism。国家自然科学基
