利用电化学固定化方法制备含辣根过氧化物酶的聚邻苯二胺（HrP/P-OPd）膜电极，改变聚合用溶液的酸度和所含支持电解质的成分，研究酶的固定化过程及其对所得酶电极性能的影响.结果表明依电聚合条件而异，酶可能以不同途径进入聚合物基质中.支持电解质的品种会影响酶膜的形成速度、组成和稳定性.所得HrP/P-OPd膜电极可在溶液不含电子传递体的条件下催化H2O2的还原，电聚合过程中进入酶膜的寡聚体可能充当酶的电子传递体By the electrochemical immobilization technique , horseradish peroxidase was entrapped into poly o phenylenediamine to prepare the enzyme membrane electrodes. The mechanism of enzyme immobilization was studied with changing the acidity of polymerization solution and the kinds of supporting electrolytes, and the properties of the resulting enzyme electrodes were examined. The results show that,depending on the immobilization conditions, the mechanism of entrapment of HRP into the polymer matrix is diFFerent.The supporting electrolytes have great inFluences on the growth rate, composition and stability of the enzyme membrane. The resulting enzyme electrode exhibits the activity to catalyze the electroreduction of hydrogen peroxide in the absence of any electron transFer mediator in solution. It was illustrated that the oliogomers ,which is produced in the course of o phenylenediamine polymerization and then incorporated in the enzyme membrane, may play a role of electron transFer mediator.国家自然科学基金;国家教委博士点基
