目的:探讨不同剂量的苯并[A]芘(b[A]P)和滴滴涕(ddT)联合暴露对小鼠血清中谷丙转氨酶(AlT)、谷草转氨酶(AST)和γ-谷胺酰转移酶(γ-gT)的影响及作用形式。方法:成年雄性昆明种小鼠50只,随机分为10组即空白对照组(正常饲养)、溶剂对照组(食用油和二甲基亚砜处理)、低和高浓度b[A]P染毒组[1.0和10 Mg/(kg·d)]、低和高浓度ddT染毒组[0.6和6 Mg/(kg·d)]、低浓度ddT+低浓度b[A]P染毒组、低浓度ddT+高浓度b[A]P染毒组、高浓度ddT+低浓度b[A]P染毒组、高浓度ddT+高浓度b[A]P染毒组。染毒组各受试物经腹腔注射染毒,每日注射1次,连续31 d。末次染毒24 H后通过眼球摘除取血,自动生化仪检测血清中AlT、AST、γ-gT的活性;并制作肝脏HE切片,观察肝细胞形态。采用两因素三水平析因设计的方差分析对数据进行统计分析。结果:与对照组比较,b[A]P和ddT单独染毒时,均能诱导AlT和AST活性升高(f=41.308,P=0.000;f=20.083,P=0.000),随各自染毒剂量的增加,AlT和AST活性升高,但二者联合暴露对AlT和AST活性均不存在交互作用(分别为P=0.258,P=0.264)。b[A]P和ddT单独染毒对γ-gT活性均未产生明显影响,联合暴露也不存在交互作用(P=0.816)。HE染色观察到肝细胞膜界限模糊,发生水样变性,肝细胞中出现小空泡,呈蜂窝状,随染毒剂量增加,肝细胞质水样变性加剧,部分肝细胞质溶解,并且细胞核肿大,形状不规则。结论:在本实验条件下,b[A]P和ddT单独染毒均能诱导小鼠血清AlT和AST活性升高,不同剂量的b[A]P和ddT联合暴露对小鼠血清AlT、AST活性的作用形式主要表现为单独作用,而非交互作用。b[A]P和ddT的单独和联合暴露均未观察到对γ-gT的活性产生明显影响。OBJECTIVE: To explore the effects of benzo[a] pyrene and DDT exposure alone or in combination on ALT,AST and γ-GT in mice.METHODS:Fifty healthy male Kunming mice were divided into 10 groups at random:blank control group(normal feed);solvent control group(equal volume of oil);1,10 mg/(kg·d) B[a]P groups;0.6,6 mg/(kg·d) DDT groups;1 mg/(kg·d) B[a]P+0.6 mg/(kg·d) DDT group;1 mg/(kg·d) B[a]P+6 mg/(kg·d) DDT group;10 mg/(kg·d) B[a]P+0.6 mg/(kg·d) DDT group and 10 mg/(kg·d) B[a]P+6 mg/(kg·d) DDT group.Exposure groups were treated with oil contained B[a]P and DDT once a day.After 31 days of intraperitoneal injection,eyeballs were extracted and ALT,AST and γ-GT were examined in blood.HE slices were made to confirm the liver damage.Using two factors and three levels factorial ANOVA design for data analysis.RESULTS:The levels of ALT and AST in all B[a]P or DDT alone exposure groups were significantly higher than that of the control groups(F=41.308,P=0.000;F=20.083,P=0.000),showing dose-effect relationships in both.But no interaction was observed in co-exposure groups(P=0.258,P=0.264).No significant changes in γ-GT were observed in all B[a]P or DDT alone exposure groups.Neither was there any interactive effect in co-exposure groups(P=0.816).This was in accordance with the observation in HE slice:hydropic degeneration,endolysis and nuclear enlargement in liver cell.CONCLUSION:Under this experimental condition,increasing levels of ALT and AST could be detected in B[a]P or DDT separate exposure groups.But neither could affect γ-GT level.No interactive effects of ALT,AST and γ-GT could be induced in co-exposure groups.福建省自然科学基金项目(2013J01354); 厦门市科技计划项目(3502Z20123003); 中央高校基本科研业务费专项资金(2011121006
