建立了快速、灵敏测定微生物油脂中花生四烯酸(AA)含量的气相色谱法(gC)。采用HP-InnOWAX毛细管柱,氢火焰离子化检测器(fId),优化了分析花生四烯酸酯化产物的色谱条件。结果表明:花生四烯酸甲酯(AA-ME)在0~1.00 Mg/Ml范围内具有较好线性,相关系数为0.999 6,检测限为0.000 5 Mg/Ml,相对标准偏差(rSd)为1.49%,回收率在95.86%~105.32%;花生四烯酸乙酯(AA-EE)在0~1.23 Mg/Ml范围内线性良好,相关系数为0.999 9,检测限为0.003 0 Mg/Ml,rSd为0.60%,回收率在97.79%~103.61%。A gas chromatography(GC) method for the determination of arachidonic acid(AA) in microbial oil was developed.Capillary column HP-INNOWAX with FID was used for detection.The parameters for analyzing esterfied AA were optimized and the method established was proved to be accurate and precise.The linearity range for methyl arachidonate was 0-1.00 mg/mL and the linear correlation coefficient was 0.999 6,the detection limit was 0.000 5 mg/mL,the relative standard deviation(RSD)was 1.49% and the recovery rate was 95.86%-105.32%.The linearity range for ethyl arachidonate was 0-1.23 mg/mL and the linear correlation coefficient was 0.999 9,the detection limit was 0.003 0 mg/mL,the RSD was 0.60% and the recovery rate was 97.79%-103.61%.厦门海洋研究开发院共建项
