分别采用甲酸+甲醛浸泡、甲醇+盐酸浸泡、丙酮浸泡、氢氧化钠浸泡、盐酸浸泡等改性方法对黑曲霉菌粉表面功能基团进行强化,以直接耐晒翠蓝fbl染料为吸附对象,通过傅里叶红外光谱仪(fT-Ir)考察浸泡处理前后吸附剂表面功能基团的结构及强度变化,探讨功能基团的强化条件。结果表明:采用盐酸+甲醇以及甲酸+甲醛浸泡处理不能提高黑曲霉菌粉的脱色能力;采用氢氧化钠浸泡处理黑曲霉菌粉的脱色能力明显提高,证明在黑曲霉吸附直接耐晒翠蓝过程中,有氨基、羟基、羧基的参与;采用盐酸浸泡处理黑曲霉菌粉表面杂质得到一定的去除,功能基团显现出来,脱色能力明显提高。The several chemical reagents, such as formic acid and formaldehyde, methanol and hydrochloric acid, acetone,sodium hydroxide, hydrochloric acid, were employed to modify the powdered Aspergillus niger, respectively, which was used for biosorption of C.I.Direct Blue 199.The structure and strength changes of functional group at the surface of powdered Aspergillus niger before and after modification were analysed by FT-IR spectrum, and the strengthen condition of the functional group was discussed.The results showed that the decolorization efficency did not changed, when the powdered Aspergillus niger were modified by hydrochloric acid and methanol, formaldehyde and formic acid, respectively.Whereas the decolorization efficency increased significantly by using the powdered Aspergillus niger modified by sodium hydroxide, implying that amino, hydroxyl, carboxyl group in the surface of powdered Aspergillus niger were involved in biosorption of C.I.Direct Blue 199.The increase in decolorization efficency was observed when the powdered Aspergillus niger were modified by hydrochloric acid, suggesting that the functional group at surface of Aspergillus niger exposed increasingly due to the removal of impurity substance at the surface of Aspergillus niger.福建省重大专项研究项目(2005YZ1023); 厦门市科技计划项目(3502Z20083001
