设计了一种可与阴道镜中的图像传感器集成的微型化多通道滤光片,以使阴道镜具有光谱成像能力。采用微光刻技术及真空多层镀膜技术制成微滤光片,内部微滤光单元的通光波长与病灶标志物反射光谱或荧光光谱的特征峰相对应,与图像传感器集成后,能够获取几幅与光谱特征峰对应的特征波长图像,这些图像凸显了病变组织与正常组织的差异,提供了宫颈组织上皮表面形态变化及上皮组织内病灶标志物含量变化的信息。已加工出通光波长为λ1=630nM、λ2=460nM、λ3=515nM、λ4=577nM的微滤光片,微滤光单元面积为10μMx10μM,各通道带宽约为40nM,透射率为30%~40%。实验显示微滤光片的光学性能已达到光谱成像的基本要求,与阴道镜集成后,能有效提高其诊断的灵敏度与特异性,减少活检频率。A micro-arrayed multichannel optical filter is proposed to allow the colposcopy to have an ability of multispectral imaging.The micro-arrayed filter is fabricated by micro-lithographic procedures and vacuum multilayer thin film coatings,and the wavelengths of light passing through the filter correspond to the characteristic peaks of the biomarkers in the reflectance or fluorescence spectra.After integrating the filter with an image sensor on colposcopy,multiple spectral images centered at different wavelengths can be obtained to provide the information about morphological changes on cervical surface and the quantitative variation of the biomarkers in cervical tissues.The micro-arrayed filter with four kinds of filter cells(λ1=630 nm,λ2=460 nm,λ3=515 nm,λ4=577 nm) has been fabricated successfully with an area of 10 μm×10 μm for each filter cell.The bandwidth of each filter cell is about 40 nm,and the transmittance is between 30% and 40%.Experiments demonstrate that the optical properties of micro-arrayed filter satisfy the basic requirements of multispectral imaging.After integrating with a colposcope,the sensitivity and specificity of colposcope diagnosis is improved significantly,which can reduce the biopsy frequencies for patients.国家自然科学基金资助项目(No.61172046);福建省自然科学基金资助项目(No.2011J01363
