本文建立了高效液相色谱串联质谱检测糖果中三聚氰胺的方法。以糖果为基质,采用三氯乙酸水溶液超声提取目标分析物,提取液经过离心、净化处理后,用强阳离子交换与反相C18混合填料色谱柱分离,乙腈和l0MMOl/l乙酸铵作为流动相(1:1),用串联质谱在多级反应监测模式下定量检测。lC-MS/MS定量限为0.01Mg/kg,线性范围为10~500μg/l,相关系数r2>0.99,平均回收率为66.8%~98.7%,相对标准偏差为1.9%~7.6%(n=6)。A method to analyze melamine residue in candies was established by using triple quadurupole liquid chromatography-tandem mass spectrometry(LC-MS/MS).Trichloroacetic acid was used to extract the target analyte.The CAPCELL PAK CR column was used for separation and the multiple reaction monitoring(MRM) positive ion mode was used for the determination.The limit of detection was 0.01mg/kg for LC-MS/MS.The coefficient of linear calibration curve was over 0.99 within the melamine concentration range of 10～500μg/L.The average recoveries of melamine by this method were 66.8%～98.7%,and the RSDs ranged from 1.9%～7.6%(n=6
