目的研究丝裂霉素C壳聚糖纳米粒(MMC-CS-nPS)对H_(22)荷瘤小鼠的肿瘤抑制作用。方法采用小鼠H_(22)肝癌细胞实体瘤模型进行体内抑瘤实验,观察荷瘤动物的生长情况,并测定肿瘤生长抑制率;肿瘤组织病理学切片行HE染色以进行形态学观察。结果 MMC-CS-nPS对小鼠的H_(22)实体瘤生长具有明显的抑制作用,低、中、高剂量组的抑瘤率分别为52.23%、66.93%、80.91%,与对照组相比有统计学意义(P<0.05)。病理组织学观察显示,各剂量组对肿瘤组织都有不同程度的破坏,肿瘤细胞大量变性坏死。结论 MMC-CS-nPS具有较强的体内抗肿瘤作用,与丝裂霉素针剂相比其抑瘤率更高,且与剂量有关,并对肿瘤生长的抑制更为持久。AIM To investigate the antitumor effect on H_(22) tumor-bearing mice treated with mitomycin C chitosan nanoparticles(MMC-CS-NPs).METHODS Hepatocellular carcinoma cell H_(22) was subcutaneously injected into mice and MMC-CS-NPs were administered to the tumor-bearing mice.The growth of tumor-bearing mice was observed,and the inhibition rate of tumor growth was calculated.The tumor tissue samples were taken and examined to assess the inhibitory effects of MMC-CS-NPs on tumor growth in the mice.RESULTS Marked inhibitory effect of MMC-CS-NPs on the transplanted hepatocellular carcinoma H_(22) was observed in the tumor-bearing mice.The inhibition rates were 52.23%,66.93%and 80.91%in the groups treated with low,medium and high dosage of MMC-CS-NPs,respectively(P < 0.05vs control group).Histopathological examination revealed widespread necrosis with massive accumulation of infiltrating lymphocytes and plasmacytes in the tumors.CONCLUSION MMC-CS-NPs has marked inhibitory effects on tumor growth in vivo.The inhibitory effects of MMC-CS-NPs are obvious and dose-dependent and higher than those of mitomycin injection.厦门市科学技术计划项目资助(编号3502Z20114007
