目的:探讨氯化三乙基锡(TETC)对体内大鼠C6胶质瘤的增殖抑制作用及病理学改变。方法:16只WISTAr大鼠皮下同种移植C6胶质瘤细胞后,随机分为实验组和对照组,实验组按1 Mg.kg-1.d-1给予腹腔注射TETC,连续给药4 d;对照组腹腔注射等量生理盐水。处理14 d后测量胶质瘤重量,计算增殖率。HE染色及电镜观察TETC作用下大鼠体内C6胶质瘤病理学变化。结果:与对照组比较,TETC实验组大鼠皮下C6胶质瘤重量减轻(P<0.01),增殖率下降(P<0.01)。HE染色及电镜观察到实验组胶质瘤细胞排列松散、细胞数目减少,可见到核染色质边集的早期凋亡改变。结论:TETC可抑制体内同种移植大鼠皮下C6胶质瘤增殖,细胞凋亡可能参与其中。Objective To study the inhibitory effects of triethyltin chloride(TETC) on proliferation and the morphological changes of rat C6 glioma cells in vivo.Methods C6 glioma cells were autotransplanted into 16 Wistar rats,and the rats were divided into experiment group and control group.TETC was injected into each rat through intraperitoneal route at the dose of 1 mg·kg-1·d-1 and the injection lasted 4 d in experiment group,and the physiological saline was injected into each rat in the same way as experiment group in control group.The weights of C6 glioma were measured and the proliferation rate of the tumor was calculated after 14 d.The morphological changes of C6 glioma cells were observed by HE staining and electron microscope.Results The weight of C6 glioma in TETC group was lighter than that in control group(P<0.01),and the proliferation rate was decreased compared with control group(P<0.01).The cell number and cell density in TETC group were lower than those in control group observed by HE staining and the chromatin margination could be observed in TETC group.Conclusion TETC can inhibit the proliferation of rat C6 glioma cells in vivo and the mechanism of which may be involved in apoptosis.国家自然科学基金资助课题(30070645
