氧化压力、缺氧及高温等不利环境条件，通常会引发生物体的压力应激反应，来应对其对机体的毒性损害以维持机体的代谢平衡。本文利用秀丽隐杆线虫为模式生物来研究14-3-3、AMPK两个保守的蛋白家族对热抗性的调节功能。前期研究结果显示，秀丽隐杆线虫中编码14-3-3蛋白的基因ftt-2在多个组织广泛表达，但仅在咽部肌肉表达ftt-2能显著延长个体寿命并增强热抗性。本文首先通过遗传学方法分析了咽部肌肉表达ftt-2热抗性增强对抗性调节因子daf-16和sir2.1的依赖性，结果显示咽部肌肉表达ftt-2热抗性增强有其他的机制。本文进一步通过RT-qPCR检测了咽部肌肉表达ftt-2转基因线虫中几个热激蛋...Environmental stress such as oxidative stress, hypoxia, and heat shock often causes proteotoxic damage,which triggers the stress-response machinery in order to maintain cellular homeostasis. In this paper, we use C. elegans as a model to study the regulatory mechanism of two conserved proteins 14-3-3 and AAK-2 on heat -resistance. Previous results showed that ftt-2 is widely expressed in many tis...学位：理学硕士院系专业：生命科学学院_细胞生物学学号：2162011115236
