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Analysis of the Response of Hepatic CYP1A in Sebastiscus marmoratus to Marine Pollution Using Loop-mediated Isothermal Amplification

Abstract

细胞色素P450(CytochromeP450)酶系的诱导已被认为是反映环境污染状况最灵敏的生物学反应之一,其中CYP1A(CytochromeP4501A)对污染物响应敏感,可作为生物标志物反映水体受原油等有机污染物污染的情况。因此,建立一种快速简便的方法检测CYP1A对环境监测具有重要意义。 环介导等温扩增法(Loop-mediatedisothermalamplification,LAMP)是一种新型的核酸扩增技术,因具有灵敏度高、特异性强、简便、快速等优点目前已广泛应用于细菌、寄生虫、病毒等方面的定性检测。 本研究建立并优化褐菖鲉(Sebastiscusmarmoratus)肝CY...Induction of cytochrome P450 enzymes has been proposed as one of the most sensitive biological response to environmental pollution. CYP1A (Cytochrome P4501A) gene is sensitive to the pollutants and it can be used as a biomarker to reflect the oil and other organic pollution in water. Therefore, it is important to establish a rapid and simple method to detect CYP1A to monitor environmental pollutio...学位:理学硕士院系专业:环境与生态学院_环境科学学号:2262011115143

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