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Studies on complete mitochondrial genome of Epinephelus coioides and Epinephelus akaara

Abstract

本文通过设计13对引物进行PCR扩增并测序,首次获得并分析斜带石斑鱼(Epinepheluscoioides)和赤点石斑鱼(Epinephelusakaara)线粒体基因组全序列,探讨石斑鱼类线粒体DNA分子进化的特点,并结合鲈形目其它种类的mtDNA进行了线粒体全基因组水平的系统发育分析。主要结果如下: 1.斜带石斑鱼和赤点石斑鱼mtDNA全序列长度分别为16418bp和16795bp,都包括13个蛋白质编码基因,2个rRNA基因,22个tRNA基因,和1个控制区。在碱基组成上都呈现显著的反G偏倚且主要表现在由H链编码的12个蛋白质基因中。2种石斑鱼的各同源蛋白质编码基因的起始密码子均相同...By designing 13 pairs of primers used in PCR and sequencing, we obtain the complete mitochondrial genome sequences of Epinephelus coioides and Epinephelus akaara.The sequences were analyzed to discuss the mtDNA evolutionary characteristics of Epinephelini, and the phylogenetic relationship of some Perciforms species. The results are shown as follows: 1. The mitogenome length of E.coioides and...学位:理学硕士院系专业:海洋与环境学院海洋学系_海洋生物学学号:20042704

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