目的：比较胰岛素生长因子-1（IGF-1）对人脐静脉平滑肌细胞（HUVSMC）和脐动脉平滑肌细胞(HUASMC)增殖的影响，并探讨其机制。 方法：本实验采用人源脐静脉平滑肌细胞（HUVSMC）和脐动脉平滑肌细胞（HUASMC）进行研究。①检测浓度效应：将HUVSMC和HUASMC各分为7组，加入不同浓度的药物。分别为：对照组、IGF-1(20ng/ml）、IGF-1(50ng/ml）、IGF-1(100ng/ml）、IGF-1(150ng/ml）、IGF-1(200ng/ml）、wortmannin（200uM）。②检测时间效应：通过浓度效应实验分析出IGF-1的合适浓度，加入IGF-1检测...Aim: To compare the influence of proliferation for the human umbilical vein smooth muscle cells (HUVSMC) and umbilical arterial smooth muscle cells (HUASMC) affected by insulin growth factor-1 (IGF-1). Methods: we used human umbilical vein smooth muscle cells (HUVSMC) and human umbilical arterial smooth muscle cells (HUASMC) for research.① to detection concentration effect:HUVSMC and HUASMC ar...学位：医学硕士院系专业：医学院临床医学系_外科学学号：2452009115299
