目的:研究骨髓间充质干细胞(mesenchymalstemcells,MSC)对A498肾癌裸鼠移植瘤的影响。方法:培养肾癌A498细胞和人MSC,用CM-Dil标记MSC并对标记后MSC细胞的活性和增殖能力进行比较,构建A498肾癌裸鼠皮下移植瘤模型,实验裸鼠共36只,当裸鼠移植瘤长径达0.5~0.8cm,将其随机分为2组:一组注射CM-Dil标记的MSC(MSC组),另一组注射生理盐水(NS对照组),其中,每组各有6只用于观察生存期。从接种肿瘤的第1天起,隔天观察裸鼠饮食、排便、活动状况以及肿瘤的生长情况;在注射MSC之后的不同时间段(3天,6天,15天,30天)用游标卡尺测量裸鼠皮下移植...Objective: to study the bone marrow mesenchymal stem cells (mesenchymal stem cells, MSC) affect A498 kidney cancer nude mouse transplantation tumor. Methods: cultivate people kidney A498 cell and MSC, use CM-Dil marker MSC and proliferation ability and activity of marker MSC cells after comparison, build A498 kidney cancer nude mice subcutaneous transplantation tumor model, a total of 36 experimen...学位:医学硕士院系专业:医学院_外科学学号:2452011115341