肝癌是一种常见的恶性肿瘤，它严重威胁着人类的健康。然而，由于肝癌本身所具有的生物学特性，使其成为目前尚缺乏有效治疗手段的疾病。苦参碱是从我国传统中药苦参的干燥根中提取出的一种生物碱活性成分，分子式为C15H24N2O，具有广泛的药理学作用。近年来的研究表明，苦参碱能在体内、外对多种肿瘤细胞起作用，诱导肿瘤细胞凋亡。然而，苦参碱诱导肿瘤细胞凋亡的作用机制还不明确。 本论文以人肝癌细胞系HepG2及裸鼠荷肝癌模型为研究对象，探讨苦参碱的抗肿瘤作用及原理。通过MTT法、PI染色法、流式细胞术，阐明了苦参碱对肝癌的增殖抑制、周期调控、诱导凋亡作用；使用WesternBlot、免疫荧光和免疫组化等方法...Hepatocellular carcinoma (HCC) is one of the common malignant tumors, and it is a serious threat to human health. However, it has not been effectively treated so far because of its biological characteristics. Matrine is one of the main alkaloid components extracted from the Sophora root, with a molecular formula of C15H24N2O. Matrine has been widely used in China, because of it has a wide range o...学位：理学硕士院系专业：医学院_药理学学号：2452010115336
