目的：研究辛二酰苯胺异羟肟酸（suberoylanilidehydroxamicacid，SAHA）对多发性骨髓瘤细胞株U266细胞增殖和凋亡的影响，并探讨其可能机制。 方法：采用台盼蓝拒染法、四氮唑蓝(MTT)比色法检测SAHA对U266细胞增殖的影响；Hoechst33342染色检测凋亡细胞的形态；AnnexinV和PI染色后应用流式细胞仪检测SAHA作用U266细胞的凋亡率；Western-blot方法检测组蛋白H3乙酰化水平、细胞周期依赖性蛋白激酶抑制剂p21蛋白表达水平以及p38MAPK信号通路相关蛋白表达水平。 结果：台盼蓝拒染法和四氮唑蓝(MTT)比色法均显示，SAHA可明显...OBJECTIVE:To investigate the effects of suberoylanilide hydroxamic acid(SAHA) on proliferation and apoptosis of multiple myeloma cell line U266 cells in vitro and explore its possible mechanism. METHODS: The cell proliferation was measured by trypan blue exclusion method and MTT assay; the apoptotic morphological change was observed with Hoechst33342 staining;the AnnexinV and PI staining was used...学位：医学硕士院系专业：医学院临床医学系_内科学学号：2452009115294
