氢是一种清洁的二次能源载体，因其环保、燃烧热值高而备受关注。生物制氢可利用微生物将有机废水、固体有机废物等转化为清洁的氢燃料，从而成为研究热点。生物制氢包括暗发酵产氢、光发酵产氢及光-暗耦合发酵产氢。在微生物体内，生物氢的产生是通过氢酶复合体来介导的，其方向是一定的。在体外，氢酶催化H22H++2e-的可逆反应，具有应用于H2/O2燃料电池、体外光解水产氢等催化过程的潜力。 产酸克雷伯氏菌HP1(KlesiellaoxytocaHP1)是一株具有耐氧产氢特性的高效产氢菌株。本文在对该菌株全基因组测序分析的基础上，构建了氢酶结构大亚基hycE及小亚基hycG过表达的重组工程菌株...Hydrogen, a clean second energy carrier, was highly concerned due to its friendly environmental character and higher combustion value. Biohydrogen has become the most advantage research field of bioenergy since it transforms the organic waste into clean fule via the microorganism. The method of biohydrogen production includes dark-fermentation, photo-fermentation and dark-photo united fermentation...学位：理学硕士院系专业：生命科学学院_微生物学学号：2162010115236
