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Experimental study on the effect of platelet-rich plasma in skin grafting

Abstract

目的 检验Landesberg改良法制备富血小板血浆的效果。观察富血小板血浆对皮片移植的存活和生长的影响,探讨富血小板血浆在创面的修复和皮片存活中的作用。 方法 以12头3个月龄左右小型猪作为实验对象,雌雄不限,体重20-25Kg。将12头小型猪随机分成3组,每组4只,分别作为术后3d组、术后7d组和术后14d组。 实验前从猪的耳缘静脉中采血,分别用Landesberg法和Landesberg改良法制备富血小板血浆,用全血细胞分析仪分别测量收集到的富血小板血浆中血小板的含量,并与全血中血小板含量做统计学比较。筛选出Landesberg改良法制备富血小板血浆的最佳方式。向制得的富血小...Objective : The purpose of this study is to examine the effects of improved Landesberg method in preparing platelet-rich plasma(PRP),observe the influence of PRP in skin surviving and growing after skin grafting,discuss the roles of PRP in wound reparing and skin surviving. Method: 12 male or female small pigs(supplied by Shanghai Slac laboratory animal CO.LTD)that were at the age of 3 month arou...学位:医学硕士院系专业:医学院临床医学系_外科学学号:2452009115296

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