苯并(a)芘(B(a)P)是研究PAHs的典型代表，具有较强的致癌性，为了研究其对海洋鱼类的生物效应，本研究以真鲷为实验动物从细胞、蛋白和分子水平上，初步探讨了B(a)P对真鲷的毒理学效应，检测了暴露B(a)P的真鲷细胞DNA损伤变化、肝脏P4501A1基因诱导表达变化以及相应蛋白酶EROD活性的变化，并比较了发生在不同水平的毒理学效应之间的相互关系，获得以下研究成果：1.克隆获得的真鲷肝P4501A1DNA、cDNA序列，序列分析表明所获得的我国真鲷P4501A1基因为鱼类P4501A1的一个同源性基因。2.用彗星实验对真鲷暴露于B(a)P三个浓度组(0.8µg/L、1.4&mi...Benzo(a)Pyrene (B(a)P) is a typical representative of PAHs which are the organic chemicals to be carcinogenic and mutagenic to animals and human. In order to assess B(a)P biological effect on the marine fish, DNA damages in cells, EROD activities and P4501A1 mRNA expression in the liver of red seabream Pagrus major exposed to B(a)P were studied using molecular techniques and comet assay. The rela...学位：工学硕士院系专业：海洋与环境学院环境科学与工程系_环境科学学号：20023401
