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Regulation of Cell Cycle and Toxin Biosynthesis in Alexandrium tamarense CI01

Abstract

塔玛亚历山大藻(Alexandriumtamarense)是一种产麻痹性贝毒(PSP)甲藻,其适应能力强、生存范围广,是重要的赤潮原因种之一。已对近海生态系统、沿海养殖业、人民健康和生命财产安全构成了严重威胁。本论文以A.tamarenseCI01为模式种,优化和完善了细胞同步化方法,并对同步化细胞进行全营养、氮限制和磷限制等大规模培养。采用细胞计数、显微观察和流式细胞DNA含量分析等手段,精确界定了不同营养条件下A.tamarenseCI01一个细胞周期内各时相的变化,研究了不同营养条件下细胞毒素含量和组成随细胞周期的变化,探讨了毒素合成与细胞周期之间的关系。同时应用蛋白质组学技术,比较研究...Alexandrium tamarense is a widely spread dinoflagellate species throughout many regions of the world, many species within this genus produces paralytic shellfish toxins (PSTs), these toxins have been negatively affecting the aquaculture industries and human health. This study optimized the synchronization method of A.tamarense CI01 cells, defined the cell cycle phases, compared toxin content...学位:工学硕士院系专业:海洋与环境学院环境科学与工程系_环境科学学号:2262006115233

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