人工雌核发育技术在育种实践和遗传学基础研究中有着广泛的应用前景，然而贝类人工雌核发育诱导至今还没有真正成功，原因之一是理化方法诱导的贝类雌核后代成活率极低。针对贝类人工雌核发育体成活率低的问题，本研究一方面细致优化了理化方法诱导杂色鲍（Haliotisdiversicolor，简为S）雌核发育程序的参数，研究了理化方法诱导杂色鲍雌核发育的细胞机理，为进一步改进杂色鲍雌核发育诱导方法、提高诱导后代成活率奠定了基础；另一方面查实了杂色鲍♀×盘鲍(H.discusdiscus，简为J)♂种间杂交F1成贝实质上是含有少量父本基因的雌核发育体，并在受精生物学和细胞遗传学研究基础上提出了杂色鲍♀×盘鲍♂产...Haliotis diversicolor (S for short) is one of most economically important mollusks cultured in the coast of Southern China. To support sustainable development of the culture industry of abalone, genetic improvement program have been proposed. Gynogenesis induction is an important chromosome set manipulation techniques with potential applications including of rapid establishment of inbred lines or ...学位：理学博士院系专业：海洋与环境学院海洋学系_海洋生物学学号：B20022700
