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Molecular Detection of Angiostrongylus cantonensis and Cloning and Expression of HSP70 Gene

Abstract

广州管圆线虫病(Angiostrongyliasis)是由广州管圆线虫(Angiostrongyluscantonensis)感染而引起的。作为一种新发的食源性传染性疾病,广州管圆线虫病在全球的流行都呈现出上升的趋势,对人类的威胁也越来越大。传统的广州管圆线虫检测对检测者的形态学知识背景要求高,且幼虫期形态很小,不易辨别。因此,建立一种操作简单、快速、灵敏度高的广州管圆线虫检测方法对广州管圆线虫病的防控很重要。 本文采用通用引物成功克隆了广州管圆线虫的rDNAITS序列(包括部分18S,ITS1,5.8S,ITS2和部分28S序列)和mtDNACOX1基因序列,并对这两个序列分别进行分析。...Angiostrongyliasis is caused by Angiostrongylus cantonensis. As a kind of newly emerged endemic disease through eating, the epidemic situation of Angiostrongyliasis is becoming more and more serious all over the world, and is shedding more and more threats on human beings. For a long time, Angiostrongylus cantonensis recovered from patients have been identified based on their morphology. However, ...学位:理学硕士院系专业:生命科学学院生物学系_动物学学号:2172008115251

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