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Studies on the sex pheromone in Chinese Black Sleeper Bostrichthys sinensis

Abstract

为了阐明性信息素在中华乌塘鳢生殖行为中的作用,本文研究了中华乌塘鳢的性信息素的种类、分泌组织和释放途径及其对异性的吸引作用。运用反相高效液相色谱(HPLC)检测性未成熟鱼和成熟鱼的生活水样、性腺提取液和尿液中的物质组成,采用Y迷宫行为实验研究了性成熟雌鱼的尿液、卵巢液和生活水对性成熟或性未成熟雄鱼的吸引作用以及性成熟雄鱼的尿液和生活水对性成熟雌鱼的吸引作用,采用质谱法对可能的性信息素物质成分进行鉴定,主要研究结果如下: 1.在运用反相高效液相色谱进行性信息素分离分析时,采用ODSC18色谱柱(4.6mm×250mm,5μm)、选择水-乙腈二元体系进行梯度洗脱、流速控制在0.5mL/min、柱...In order to clarify the roles of the sex pheromone in reproductive behaviors in Chinese black sleeper (Bostrichthys sinensi), the kinds of the sex pheromones and their secretive tissues,release routeways and attraction to opposite sexes were investigated. The composition of the holding water, gonadal extraction and urine from mature and immature Bostrichthys sinensi was examined using reverse-phas...学位:理学硕士院系专业:海洋与环境学院海洋学系_海洋生物学学号:2242006115231

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