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Proteomic Analysis of Cellular Response to Anti-peptide from Meretrix meretrix Linnaeus in hepatocarcinoma SMMC-7721 Cells

Abstract

文蛤(学名:MeretrixMeretrixLinnaeus,英文名:Hardclam)不仅肉质鲜美、营养丰富,而且具有很高的药用价值。据报道文蛤具有抗癌、降血糖、降血脂和抗氧化等活性。许多研究者已对文蛤抗癌活性做了研究,证实文蛤提取物具有较强的抗癌活性。 将文蛤肉匀浆、抽提,并通过G-25凝胶层析柱分离,得到具有抑癌活性的组分,初步判断为肽类物质。该组分在浓度为0.5μg/mL时对肝癌细胞SMMC-7721的抑制率能达到50.6%。通过光学显微镜观察,可以发现肝癌细胞在文蛤提取物作用后形态发生很大变化。 利用蛋白质组学手段研究文蛤活性多肽对肝癌细胞的作用有助于更好地研究该物质抑制肝癌细胞...Meretrix meretrix Linnaeus is smooth-shelled marine mussels.It not only is delicious but has medicinal properties. Some researchers have reported that Meretrix meretrix Linnaeus can lower blood sugar and lipid. It is also resistant to oxidation and has immunocompetence. Now more and more researchers are intresting in study of anticancer active of extract from Meretrix meretrix Linnaeus. We pu...学位:理学硕士院系专业:生命科学学院生物化学与生物技术系_生物化学与分子生物学学号:20042610

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