Isolation of microsatellite markers, construction of genetic linkage maps and QTL mapping of growth-related traits in small abalone, Haliotis diversicolor
本研究采用磁珠富集法，用14个探针构建了杂色鲍微卫星富集文库。从文库中随机选取768个阳性克隆进行测序，拼接得到729个重叠群，其中656个重叠群包含微卫星序列。利用微卫星两侧保守区域设计了583对微卫星引物，从中筛选出退火温度在50-64℃，PCR产物稳定扩增、条带清晰的微卫星标记308个。随机选取其中的11个微卫星标记用于标记质量评估，这11个微卫星标记的PIC值在0.7276到0.9163之间，具有高度多态性。 利用开发的308个微卫星标记，以养殖个体♀和野生个体♂杂交F1家系为作图群体，构建了杂色鲍的遗传连锁图谱。308个微卫星标记中有182个标记在亲本中具有多态性，可以用于图谱构建...Small abalone, Haliotis diversicolor is naturally distributed along the coastal waters of East Asia: from Japan to the Philippines. It is an economically important maricultured species in southern China and Taiwan. The molecular genetic likage map of small abalone is essential for the identification and location of quantitative trait loci (QTL) as well as for the marker-assisted selection program,...学位：理学博士院系专业：海洋与环境学院海洋学系_海洋生物学学号：BH170022
