目的体外组织培养法诱导羊膜基质细胞向神经细胞分化。 方法将新鲜去上皮羊膜（dAM-0W）和完整羊膜（iAM-0W）固定于特制的培养插件(insert)上进行组织培养，采用神经细胞分化培养基诱导羊膜基质细胞的分化。在培养1周、4周时进行Hoechst染色，观察基质细胞的增殖情况。取新鲜羊膜（dAM-0W）、培养4周的去上皮羊膜（dAM-4W）及完整羊膜（iAM-4W），分别提取RNA进行实时荧光定量PCR检测、制备组织块进行免疫荧光染色、提取蛋白质进行WesternBlot印迹检测，以分析基质细胞起源的神经细胞标记物的表达情况。 结果Hoechst染色结果显示：培养4周的去上皮羊膜（dAM-...Objective To induce amniotic membrane (AM) stromal cells differentiate into neural cells using ex vivo tissue culture method. Methods Fresh denuded AM (dAM-0W) and fresh intact AM (iAM-0W) were mounted on special designed inserts and cultured in neural differentiation medium for different durations up to 4 weeks. Hoechst staining was performed to investigate cell proliferation. Real time PCR, imm...学位：理学硕士院系专业：医学院基础医学系_微生物学学号：2452009115301
