Cloning and prokaryotic expression of the main subunit allophycocyanin genes from Porphyra haitanensis

Abstract

别藻蓝蛋白是一种藻胆蛋白,作为捕光和激发能传递物质,在藻类的光合作用中起重要的作用;作为储存蛋白,是一种价值很高的营养成分。不仅如此,藻胆蛋白在免疫荧光技术、抗肿瘤药物开发、医疗保健药物开发、食品工业、精细化工和化妆品工业等许多方面有很重要的应用价值和研究前景。别藻蓝蛋白主要由α、β两亚基聚集构成,每个亚基上均含有一个藻蓝胆素分子。别藻蓝蛋白α、β两亚基分别由叶绿体基因组编码,并且两亚基基因相毗连,中间只有少数碱基的间隔序列。 本课题以阴干的坛紫菜叶状体为材料,通过酶解等方法获取完整的叶绿体,再裂解叶绿体并酚仿抽提获得叶绿体DNA。限制性内切酶HindIII和EcoRI双酶切及RAPD检测结...Allophycocyanins, one of phycobiliproteins, acted as substances of light-havesting and transferring energy to photosystem reaction centers in algal photosynthesis;also as a kind of valuable nutritious components as store proteins;moreover,many researches revealed that allophycocya- nins had extensive applied perspective in quite a lot of fields,such as immunofluorescence technique, anti-tumour med...学位:理学硕士院系专业:生命科学学院生物学系_动物学学号:20002601

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