摘要纤维素资源的开发与利用一直是国内外研究的热点，而纤维素的高效降解与糖化是制约纤维素生物质应用的关键。本研究从腐烂稻草、朽木条、土壤和牛粪等样品中分离到6株纤维素降解菌株。通过滤纸崩解试验、刚果红纤维素平板识别以及液体发酵产酶鉴定，筛选到一株分解纤维素能力较强的真菌。经形态观察和18SrDNA基因片断分析，鉴定该菌株为青霉T24-2。对青霉T24-2的液态发酵条件进行研究。菌株在含3％稻草粉、0.25％尿素和无机盐营养液的培养基中发酵4d,自然pH，30℃，130r/min,菌株的CMC酶活（羧甲基纤维素酶活）和滤纸酶活分别达到45.01IU/mL和6.89IU/mL。采用液态发酵法对该菌酶...Abstract The search and exploitation of efficient cellulolytic strains is the key step for the exploiture and utilization of cellulose resources. In order to obtain efficient cellulose decomposing microbes, the author isolated 6 cellulose pro- ducing strains from putrid straw, wood, soil, and cattle feces etc. On the basis of these strains, a high cellulase-producing strain was isolated through th...学位：理学硕士院系专业：生命科学学院生物学系_微生物学学号：20042603
