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Optimization of fermentation parameters for heterotrophic growth and DHA accumulation of Schizochytrium sp. TIO1101 and physiology-biochemistry characteristics of transgenic Schizochytrium

Abstract

二十二碳六烯酸(Docosahexaenoicacid,DHA)是一种具有重要生理意义和经济价值的高不饱和脂肪酸,其传统的制备方法是从深海鱼油中提取。但是,深海鱼油因鱼类资源供应限制,DHA含量低,提取得到的DHA有鱼腥味等缺点,难以满足市场需求。因此,开发新的DHA来源已成为相关研究的重点。裂壶藻是一种异养海洋微藻,具有不饱和脂肪酸组成简单、DHA含量高、易于培养、对人畜无毒害等优点,成为近年来DHA工业化生产领域研究的热点。 本论文利用本实验室分离自红树林的一株裂壶藻藻株Schizochytriumsp.TIO1101,研究了培养基组分、培养时间和环境因子对其生长和DHA含量的影响;在选...DHA (Docosahexaenoic acid) is a kind of polyunsaturated fatty acids with vital physiological functions and high economic values. More and more attention has been paid on production and preparation process of DHA, However, conventional fish oils may not be suitable to meet the increasing demand for DHA owing to their limited supply, lower content of DHA and peculiar taste and odor. Schizochytrium s...学位:理学硕士院系专业:生命科学学院生物学系_生物化学与分子生物学学号:2172009115206

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