放线菌的次级代谢产物是一类重要的微生物药物资源。近年来,选择性分离放线菌的方法学研究取得了显著成绩。基因筛选、培养基筛选、原位筛选等成为当今研究的重要方法。虽然这些分离方法出发点不同,但是它们都为放线菌的新药发现提供了宝贵的菌种资源。 本论文以厦门、云南、青海三地的植物根际土壤样品作为实验材料,采用了聚乳酸(Poly(L-lactide))(PLA)-明胶培养基用于稀有放线菌的分离。通过选择分离能力的比较发现,聚乳酸(PLA)-明胶培养基比腐殖酸培养基有更好的选择分离效果。利用聚乳酸(PLA)-明胶培养基从11份根际土壤中总共分离得到85株放线菌,根据16SrRNA鉴定获得除链霉菌属之外的稀...There are many nature products were separated from actinomycetes. Nature products are the major source of clinical medicine, so actinomycetes’ secondary metabolites have been considered as important microorganism resources. Lately, selective isolation methods have made great progress, especially in methods of gene-mining, selective media, screening in situ and so on. Although all these methods hav...学位:理学硕士院系专业:生命科学学院生物化学与生物技术系_微生物学学号:2172009115214