Isolation and screening of Bacillus thuringiensis from South China Sea and Preliminary Studies on Chitinase- producing Conditions and Identification of a Chitin-degrading strain
苏云金芽孢杆菌（Bt）是目前世界上应用范围最广的杀虫微生物，它产生的杀虫晶体蛋白是巨大的天然害虫生防资源，对多个目的害虫具有杀虫活性；而几丁质酶（chitinase）作为一种重要的杀虫活性物质，具有多种富有价值的生物学功能，它们在生物防治、环境保护等方面都具有重要的社会效益和经济价值。因此，开展新型苏云金芽孢杆菌资源的分离筛选、鉴定、分子生物学以及产几丁质酶相关条件的研究都具有重要意义。 海洋环境的特殊性往往造就了海洋微生物及其代谢产物的多样性，因而是人们寻找与发现活性物质产生菌的重要场所。本研究采用醋酸钠法加热处理，对南海吕宋、越南上升流区所采集的水体和沉积物样品进行了分离筛选，共得到22...Bacillus thuringiensis (Bt) is one of the most widely used insecticidal microbe. Insecticidal crystal proteins (delta-endotoxins) from Bt, which are toxic to pests from many insect orders, are great biocontrol resources against natural pests. As an important kind of insecticidal active substances, chitinase has many valuable biological funtions.Both of them are very valuable in many areas, such as...学位：理学硕士院系专业：生命科学学院生物学系_微生物学学号：2012005130202
