本文采用乙醇抽提、分子筛层析等方法从文蛤（MeretrixMeretrix）中提取到具有抗癌活性物质，将它命名为MM2。成分分析显示其中含糖4.82mg/mL，蛋白2.08mg/mL，质谱测定其分子量为3987.7Da，推测其可能是一种糖肽。MTT结果显示MM2对Hela细胞增殖生长有显著的抑制作用，且呈时间和剂量效应。采用Giemsa、Hoechst、AO/EB染色及透射电镜等细胞形态观察，发现MM2处理后Hela细胞形态发生明显改变，轮廓变得不清晰，细胞皱缩变圆，连接减少，细胞脱壁而至悬浮状态；Giemsa染色可见核处于溶解状态，核仁消失，染色质高度凝集且边缘化；Hoechst染色可见致密...In our study, we purified the active component from Meretrix Meretrix Linnaeus, named it as MM2, with a series of methods including, organic extraction and column chromatogram. Component analysis showed that the extract contained sugar and peptide simultaneously, the concentration of them were 4.82 mg/mL and 2.08 mg/mL, respectively,with 3987.7 KD molecular weight. The results showed that the acti...学位：理学硕士院系专业：生命科学学院生物化学与生物技术系_生物化学与分子生物学学号：2162007115202
