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Preparation and Immunoassay of Monoclonal Antibodies against Bioactive Paeoniflorin of Chinese Materia Medica

Abstract

分子生物学等生物技术的快速发展,为中药的研究开辟了许多新的途径。其中,应用小分子量中药活性成分的单克隆抗体(monoclonalantibodies,MAbs)建立的免疫测定系统已成为受体结合分析、酶测定等研究的一个重要工具,也是药用植物的一种定性定量分析技术,这些都与MAbs的特异亲和力、可大量生产等相关。但是,单克隆抗体用于现代中医药的研发方面的文献至今尚所见不多,因此,亟需探索有关单抗技术在中医药研究中的技术基础及其应用。本研究的目的就是通过制备芍药的主要活性成分—芍药苷(paeoniflorin,PF)的单克隆抗体,从而建立以此为基础的酶联免疫测定(enzyme-linkedimmun...Immunoassay systems using monoclonal antibodies (MAbs) against naturally occurring bioactive compounds having lower molecular weights have become one of important tools for the studies on receptor binding analysis, enzyme assay, and qualitative and/or quantitative analysis techniques in herbal medicines owing to their specific affinity. However, there are not many reports and references about the ...学位:理学硕士院系专业:生命科学学院生物学系_细胞生物学学号:2012005130207

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