水华是在一定的营养、气候、水文条件和生物环境下形成的藻类过度繁殖和聚集的现象,它的生消与营养、水温、光照、O2等环境密切相关。水华暴发的后期,由于藻类和细菌微生物的大量繁殖,使水体耗氧量大大增加,导致水体缺氧,引发蓝藻的大面积死亡。据推测,这是一种细胞程序性死亡(PCD)。 为了验证这一推测,我们以铜绿微囊藻PCC7806为研究材料,模拟水华后期水体缺氧的状态,利用光生物反应器给予藻黑暗断气处理,阻断其O2的供应,同时,以100mmol/L双氧水诱导藻的PCD作为阳性对照,分别在胞质水平上对细胞内活性氧(ROS)、半胱氨酸蛋白酶(Caspase3)活力进行检测;在脱氧核糖核酸(DNA)水平上...Algal bloom is a rapid increase or accumulation in the population of algae in a certain nutrients, climate, hydrological conditions and biological environment. It is closely related to nutrients, water temperature, light, oxygen and some other environmental factors. In the later period, massive algae die because of mass propagation of algae and bacteria, which increases oxygen consumption of water...学位:理学硕士院系专业:生命科学学院生物学系_生物化学与分子生物学学号:2162007115189