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Identification of the Envelope Protein Genes and Primary Characterization of the Protein Binding to the Homologous Repeat Regions of White Spot Syndrome Virus

Abstract

对虾白斑综合症病毒(whitespotsyndromevirus,WSSV)是对虾的最主要病毒病原之一。它的宿主范围广,传染力强,致死率高,难防治,不仅严重危害对虾养殖,还对海洋环境构成威胁。本论文开展了病毒膜蛋白以及可能与病毒复制或调控相关的蛋白基因的研究,有利于揭示WSSV入侵的分子途径,探索病毒复制或转录的关键蛋白,为最终建立有效的防治方法提供有力的科学依据。从感染螯虾(Procambarusclarkii)组织中大量提取和纯化了完整的白斑综合症病毒粒子。病毒膜蛋白组份经SDS-PAGE和质谱分析,发现三个分子量为124kDa、39kDa和187kDa的蛋白分别是WSSV基因组的开放阅读...White spot syndrome virus (WSSV) is one of the most virulent pathogens in shrimp culture worldwide. It has a wide range of hosts in crustaceans,a high infection and mortality rate, and is hard to be controlled. Therefore WSSV is not only a major threat to the shrimp industry but also to the marine environment everywhere. In this thesis, some genes related with viral envelope proteins or possibly i...学位:理学博士院系专业:生命科学学院生物学系_生物化学与分子生物学学号:B20022601

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