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Characterization of an envelope protein(VP110)of shrimp white spot syndrome virus

Abstract

对虾白斑综合症病毒(whitespotsyndromevirus,WSSV)是一种具有囊膜的、无包涵体的、类杆状双链环状DNA病毒,是危害对虾养殖的主要病原之一,对世界对虾养殖业造成了严重的损失。本论文根据实验室已建立的大量纯化完整WSSV病毒粒子的方法,利用SDS-PAGE电泳结合蛋白质质谱技术对WSSV膜蛋白和核衣壳组分进行了初步分离鉴定,质谱结果分析发现在膜蛋白组分中有一分子量在110kDa的蛋白条带与WSSV基因组中WSV035阅读框的编码蛋白相匹配(因此称之为VP110),推测该蛋白可能为病毒的膜蛋白。WSV035(称之为vp110)全长2916bp,编码972个氨基酸残基,预计分子...White spot syndrome virus (WSSV),an enveloped, non-occluded, bacilliform and circular dsDNA virus, is one of the major pathogens of shrimp diseases, causing considerable mortality in penaeid shrimp. Based on the efficent method for purification of intact WSSV viral particles constructed in our lab, envelope proteins and the nucleocapsid fraction of WSSV were seperated by SDS-PAGE and further i...学位:理学硕士院系专业:生命科学学院生物学系_细胞生物学学号:20012604

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