对虾白斑综合症病毒（WhiteSpotSyndromeVirus，WSSV）是危害对虾养殖业的最主要病原，它是一种具有囊膜、无包涵体的杆状型双链环状DNA病毒。病毒极早期基因编码的调控蛋白在病毒的感染过程中起着至关重要的作用。本文阐述了在体外原代培养的螯虾血淋巴细胞中WSSV基因转录和基因组复制的情况。前期的研究工作成功的构建了基于原代螯虾血淋巴细胞体外培养的WSSV感染模型；利用WSSV全基因组芯片杂交技术对用蛋白抑制剂-放线菌酮（CHX）处理的原代培养的螯虾血淋巴细胞的样品进行WSSV极早期基因的筛选。本文对芯片筛选得到的候选基因进行RT-PCR鉴定和转录时相分析，最终确定了16个WSSV...White Spot syndrome virus (WSSV) is the major viral pathogen of shrimp industry, which is kind of enveloped, no inclusion stage, rod-shaped, double-strand DNA virus. During viral infection, viral immediate-early (IE) genes encode regulatory proteins critical for the viral life cycle. This thesis demonstrated WSSV genes’ expression and genome replication in primary cultured crayfish hemocyte in vit...学位：理学硕士院系专业：生命科学学院生物学系_生物化学与分子生物学学号：2162007115192
