N-乙酰-β-D-氨基葡萄糖苷酶(N-Acetyl-β-D-glucosaminidase，简称NAGase，EC.3.2.1.52)在南美白对虾(Penaeusvannamei)的蜕壳发育和营养代谢中起重要作用，且具有多种应用价值。本文以南美白对虾内脏为提酶材料，获得电泳单一纯的NAGase酶制剂，本文以此酶制剂为对象展开研究。常见金属离子Li+、Na+、K+对酶活力没有影响，Co2+、Cd2+、Ba2+、Zn2+、Al3+、Pb2+、Cu2+、Fe3+、Ag+、Hg2+，有不同程度的抑制。Ag+离子对酶表现为反竞争性抑制（KIS为2.39mmol/L），Zn2+离子对酶表现为非竞争性抑制（...N-Acetyl-β-D-glucosaminidase (NAGase, EC3.2.1.52) plays an important role in molting and nutrition assimilation of Penaeus vannamei, and uses in other applications. The NAGase was purified from viscera of Prawn (Penaeus vannamei) and determined to be homogeneous by polyacrylamide gel electrophoresis (PAGE) and SDS-PAGE. This purfied enzyme will be used in the following studies. The effects ...学位：理学硕士院系专业：生命科学学院生物学系_生物化学与分子生物学学号：20032609
