本文以五唇兰、蝴蝶兰为材料，应用石蜡切片和花粉压片观察了2种兰花雄配子体的发育，进行了精细胞的分离；在分离生活胚囊的基础上，进行了卵细胞、合子和多细胞胚的分离研究。本文的主要结论是：1.五唇兰、蝴蝶兰的小孢子四分体均有四面体型、左右对称形、交叉形和T形等多种类型，它们发育成四合花粉，并由四合花粉聚集成花粉块。花粉成熟时为二细胞型。2.通过人工授粉使花粉管在子房内发育，再利用花粉管在甘露醇中直接爆破分离出五唇兰、蝴蝶兰的精细胞，成对的2个精细胞在体积大小、荧光强弱均显示有较大差异。应用显微操作仪可收集精细胞群体。3.应用酶解-振荡法处理胚珠，分离出2种兰花的生活胚囊，继之以显微解剖分离出2种兰花...Based on the materials of Doritis pulcherrima and Phalaenopsis hybrid， the development of male gametophytes were observed by the methods of paraffin sections and pollen compression. The isolation of sperm cells, living embryo sacs, eggo cells, zygotes, and proembryos in these two kinds of orchid were studied. Results presented in this thesis can be summarized as follows: -The microspore tetrads ...学位：博士后院系专业：生命科学学院生物学系_发育生物学学号：BH1700017
