目的: 构建由癌胚抗原启动子(CEApromoter，Cp)驱动的双自杀基因CD-TK治疗载体pcDNA3.1(-)Cp-CD-TK，研究癌胚抗原启动子能否控制CD-TK基因在CEA阳性大肠癌细胞中专一性表达和杀伤，比较融合基因与单基因对肿瘤细胞生长抑制方面有无差异，是否存在协同效应，并确定安全有效的药物使用浓度。观察旁观者效应。 方法: 分别以提取的人血细胞基因组DNA，pMD18-CD质粒和tgCMV/Hy-TK质粒为模板，PCR扩增Cp、CD和TK基因片段，采用双酶切、连接等方法依次将Cp、CD、TK基因亚克隆到载体pcDNA3.1(-)，构建含Cp启动子和CD-TK融合基因的...Objective: To construct targeted plasmid vector pcDNA3.1(-)Cp-CD-TK which contains CEA promoter. To study CD-TK expression driven by CEA specific promoter and its killing effect on CEA-positive colorectal carcinoma cells. By comparing the tumor growth inhibition effects among fusion gene and individual genes, find out whether there is co-effect; find out the optimum concentration; and observe th...学位：理学硕士院系专业：生命科学学院生物学系_生物化学与分子生物学学号：2172006115222
