摘要 ArmoredRNA技术是近几年发展起来的一种新的RNA质控品的制备技术。该技术的原理就是利用基因工程方法将包含有大肠杆菌MS2噬菌体的外壳蛋白基因的序列及外源片段克隆到表达载体中,这一载体能够将外源克隆片段转录成重组RNA,并利用载体上MS2的外壳蛋白基因合成的外壳蛋白将其装配成球状RNA病毒结构的RNA-蛋白质复合体,我们称之为RNA病毒样颗粒。目前ArmoredRNA技术正逐渐应用于RNA病毒的检测方面。 首先,根据ArmoredRNA技术的原理,构建了能表达耐核糖核酸酶病毒样颗粒的载体,选取口蹄疫病毒IRESRNA片断为内标检测基因,克隆到表达载体噬菌体基因的下游,最终得到能...ABSTRACT Armored RNA technology is a new technique of preparation for RNA quality control, which is developed in recent years. The principle is that E.coli bacteriophage MS2 coat protein gene and foreign fragment was cloned into the expression vector by genetic engineering methods, and the foreign fragment can be transcripted into recombinant RNA. The recombinant RNA is packaged by MS2 coat prote...学位:理学硕士院系专业:生命科学学院生物化学与生物技术系_细胞生物学学号:20042607