Mass Characteristics and Stability of the Ferritin and the Polysaccharide Nanoparticles of Insulin with MALDI-TOF Mass Spectrometry

Abstract

铁蛋白是一种最常见的水溶性蛋白质,它不仅具有储存和释放铁的生理功能,而且与人类及动物的各种疾病的形成和发展有着不可分割的联系。深入研究铁蛋白的结构与功能,其分析技术和科研成果将对诊断和治疗人类各种疾病起着极其重要的作用。拓展铁蛋白新功能,可服务于医学科学、环境与工程和材料科学等其它研究领域,研究意义重大。 本研究从魟鱼肝中分离出魟鱼肝铁蛋白(LiverferitinofDasyatisakajei,DALF),根据DALF耐热性特点,采用热变性技术、离子交换层析技术(DEAE-52)、分子筛层析技术(G-25、SephacrylS-300)和聚丙烯酰胺凝胶电泳技术制备电泳纯铁蛋白和不同多聚体...Ferritin is the most common hydrophilic iron-storage protein. Ferritin not only plays very important functions in organisms, but also has very direct relations with many kinds of diseases. So studies on the functions and structure of ferritin can lead to diagnosis and treatment of human diseases and development of ferritin to be used in medicine, environment science, engineering and material scien...学位:理学硕士院系专业:生命科学学院生物学系_生物化学与分子生物学学号:20002603

    Similar works