本研究应用从中国鲎血细胞中分离提取的鲎素处理人肺腺癌SPC-A-1细胞和人胚肺二倍体成纤维WI-38细胞，观察和鉴定鲎素对SPC-A-1细胞的生物学效应及其作用性质，并进一步探索鲎素对肺癌细胞的作用机制。 实验结果表明，6.0μg/ml的鲎素能有效地抑制SPC-A-1细胞增殖活动，细胞生长抑制率达78.64%，分裂指数峰值下降至27‰，细胞周期发生明显阻滞，G0/G1期细胞比例增加，S期细胞比例下降。光镜、扫描和透射电镜观察结果显示，经鲎素处理后的SPC-A-1细胞形态和结构发生了显著的变化，表现出与相同来源正常细胞相似的结构特点：细胞形态较为一致，细胞体积增大；细胞核变小且形状较为规则，核...In the study, we used the tachyplesin which is isolated and purified from the acid extracts of horseshoe crab (Tachypleus tridentatus) hemocytes, to treat the human lung adenocarcinoma SPC-A-1 cells and the human normal diploid cell line WI-38 , and investigated the biological effects of tachyplesin on SPC-A-1 cells and functional characteristics, and further study the functional mechanism of tach...学位：理学硕士院系专业：生命科学学院生物学系_细胞生物学学号：19982601
