对虾白斑综合症病毒(WSSV)是困扰对虾养殖业最主要的病原体,目前人们对它的侵染机制还不完全的了解。与其他DNA病毒相似,WSSV的基因转录通常具有时相性的特征,根据转录时间的不同可以细分为极早期基因、早期基因、晚期基因。本研究选出其中三个极早期基因wsv051,wsv056,wsv083进行研究,5`RACE实验具体找到这三个极早期基因的起始转录位点,发现wsv051的起始转录位点位于ATG上游366nt处,wsv056的起始转录位点位于ATG上游227nt处,wsv083的起始转录位点位于ATG上游113nt处。wsv051,wsv083的起始转录位点位于预测的TATAbox下游23nt处...White Spot syndrome virus (WSSV) is currently the major viral pathogen of aquacultured shrimps that causes huge economic losses to shrimp farming industries worldwide. However, the mechanism of WSSV infection is not fully understood. Like most dsDNA virus, WSSV transcribes its genes in a temporal manner, which is classified into three stages: immediate-early (IE),early, and late. This study is foc...学位:理学硕士院系专业:生命科学学院生物学系_生物化学与分子生物学学号:2172008115262