lysA基因编码二氨基庚二酸脱羧酶（DAPDC)，该酶将二氨基庚二酸（DAP）转化为L－Lys，因此DAPDC对细菌的生长和生存至关重要。本实验从盐生杜氏藻高盐培养液中分离纯化了一株盐单胞菌，并从形态、生理以及16SrDNA等方面进行初步鉴定，将它命名为Halomonassp.ZL-4。 采用鸟枪法构建了Halomonassp.ZL-4部分基因组文库。提取Halomonassp.ZL-4的总DNA，经限制性核酸内切酶Sau3AI消化后回收1~6kb大小的酶切片段，与经限制性核酸内切酶BamHI酶切消化、回收后的质粒pUC19连接，热转化感受态细胞E.coliDH5α。在此基础上，进行克隆子的...DAPDC is a vitamin B6-dependent enzyme that stereospecifically converts meso-DAP to L-lysine. The enzyme is of interest because of its importance in bacterial growth and survival. Lysine is essentially required in protein biosynthesis and for their viability and development. So biosynthesis of L-lysine has come under increased scrutiny as a target for novel antibacterial agents. A strain of h...学位：理学硕士院系专业：生命科学学院生物化学与生物技术系_水生生物学学号：20042603
